Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

Article Title: Power-free microchip immunoassay of PSA in human serum for point-of-care testing.

doi: 10.2116/analsci.27.237

Figure Lengend Snippet: Fig. 2 Time course of the fluorescent intensity of the microfluidic immunoassay. The 0 min of amplification time in the horizontal axis is defined as the time from which both F-SA and b-anti-SA start flowing into the microchannel. PSA was diluted with PBS containing 1% BSA (a-1 and a-2) or human female serum (b-1 and b-2). Each data point is the average of four measurements obtained by four independent experiments. Additionally, fluorescence was obtained from LFDA (a-1 and b-1) or from no amplification (a-2 and b-2). The concentrations of PSA: blank (▼), 4.0 ng/mL (△), 100 ng/mL (■), 1.0 μg/mL (×), and 10 μg/mL (◣).

Article Snippet: Sample solutions of PSA were created by diluting PSA (Acris Antibody, Herford, Germany) either in PBS (pH 7.5, Cambrex Biosciences, Walkersville, MD) or in human female serum (Scipac, Kent, UK) to the desired concentrations.

Techniques: Amplification, Fluorescence

Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

Article Title: Power-free microchip immunoassay of PSA in human serum for point-of-care testing.

doi: 10.2116/analsci.27.237

Figure Lengend Snippet: Fig. 3 Calibration curve of PSA diluted in PBS (a-1 and a-2) or human female serum (b-1 and b-2). The error bars represent one standard deviation of four independent experiments. (a-1 or b-1) The curves were obtained from LFDA and the amplification time was 7.0 min. Insets magnify the graphs of the low concentration range. (a-2 and b-2) Calibration curve of PSA without amplification as control experiments. The time of detection was 4.0 min after the start of flow of F-SA.

Article Snippet: Sample solutions of PSA were created by diluting PSA (Acris Antibody, Herford, Germany) either in PBS (pH 7.5, Cambrex Biosciences, Walkersville, MD) or in human female serum (Scipac, Kent, UK) to the desired concentrations.

Techniques: Standard Deviation, Amplification, Concentration Assay, Control

Journal: Technology in Cancer Research & Treatment

Article Title: Deletion of NKX3.1 via CRISPR/Cas9 Induces Prostatic Intraepithelial Neoplasia in C57BL/6 Mice

doi: 10.1177/1533033820964425

Figure Lengend Snippet: Level of PSA and apoptotic proteins. (A) The PSA concentration was measured in the prostate homogenate by enzyme-linked immunosorbent assay. The detection ranges of the kit used is 0.312-20 ng/mL. Five to 6 mice per group were used for preparing the protein lysate, and ELISA were assayed in duplicate for each sample. (B) The expression of Bax and Bcl-2 proteins were measured by western blot analysis using HRP-labeled anti-rabbit IgG antibody. After intensity of each band was determined using an imaging densitometer, the relative levels of the 2 proteins were calculated based on the intensity of β-actin. Five to 6 mice per group were used for preparing the protein homogenate, and western blot analyses were assayed in duplicate for each sample. Data are reported as the mean ± SD. *, p < 0.05 compared to the WT mice. #, p < 0.05 compared to the HT mice.

Article Snippet: The level of PSA was quantified using ELISA kits (CSB-E08276m, Cusabio Biotech Co., Ltd., Wuhan, China), according to the manufacturer’s instructions.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Labeling, Imaging

Journal: Cancer Management and Research

Article Title: Normal Basal Epithelial Cells Stimulate the Migration and Invasion of Prostate Cancer Cell RM-1 by TGF-β1/STAT3 Axis in vitro

doi: 10.2147/CMAR.S303122

Figure Lengend Snippet: Identification of basal epithelial cells acquired from mouse prostate with anti-p63 in immunofluorescence assay (Objective 10X). Basal epithelial cells were specific to anti-p63 and more than 90%.

Article Snippet: Mouse prostate cancer cells RM-1 and mouse primary normal prostate basal epithelial cells were obtained from iCell Bioscience Inc. in Shanghai.

Techniques: Immunofluorescence

Journal: Cancer Management and Research

Article Title: Normal Basal Epithelial Cells Stimulate the Migration and Invasion of Prostate Cancer Cell RM-1 by TGF-β1/STAT3 Axis in vitro

doi: 10.2147/CMAR.S303122

Figure Lengend Snippet: Normal basal epithelial cells could stimulate the migration of RM-1 directly or indirectly. ( A ) Effect of basal epithelial cells on cells wound healing of RM-1 was determined after being mixed-cultured (1:1) for 0 h and 24 h. ( B ) Quantification of migrated width of wound healing assay. ( C ) Effect of basal epithelial cells on migration of RM-1 was determined after being co-cultured for 20 h. ( D ) Quantification of migrated cells in ( C ). ( E ) Effect of 10% conditional medium of basal epithelial cells on migration of RM-1 was estimated after being cultured for 20 h. ( F ) Quantification of migrated cells in (E). Values are represented by mean±SD from at least three independent experiments. “*” represents “p<0.05”, “***” represents “p<0.001” vs control group.

Article Snippet: Mouse prostate cancer cells RM-1 and mouse primary normal prostate basal epithelial cells were obtained from iCell Bioscience Inc. in Shanghai.

Techniques: Migration, Cell Culture, Wound Healing Assay, Control

Journal: Cancer Management and Research

Article Title: Normal Basal Epithelial Cells Stimulate the Migration and Invasion of Prostate Cancer Cell RM-1 by TGF-β1/STAT3 Axis in vitro

doi: 10.2147/CMAR.S303122

Figure Lengend Snippet: Normal basal epithelial cell promoted the invasion of RM-1 directly or indirectly. ( A ) Effect of basal epithelial cells on invasion of RM-1 was determined after being co-cultured for 20 h. ( B ) Quantification of invasive cells in ( A ). ( C ) Effect of 10% conditional medium of basal epithelial cells on invasion of RM-1 was estimated after being cultured for 20 h. ( D ) Quantification of invasive cells in ( C ). Values are represented by mean±SD from at least three independent experiments. “**” represents “p<0.01”, “***” represents “p<0.001” vs control group.

Article Snippet: Mouse prostate cancer cells RM-1 and mouse primary normal prostate basal epithelial cells were obtained from iCell Bioscience Inc. in Shanghai.

Techniques: Cell Culture, Control

Journal: Cancer Management and Research

Article Title: Normal Basal Epithelial Cells Stimulate the Migration and Invasion of Prostate Cancer Cell RM-1 by TGF-β1/STAT3 Axis in vitro

doi: 10.2147/CMAR.S303122

Figure Lengend Snippet: Basal epithelial cells secreted TGF-β1 and additional TGF-β1 stimulated the migration and invasion of RM-1. ( A ) Quantification of TGF-β1 levels measurement in ELISA assay. Effect of 5 ng/mL TGF-β1 on migration ( B ) and invasion ( C ) of RM-1 in transwell chamber with or without matrigel after being cultured for 20 h. ( D ) Quantification of invasive cells in ( C ). Values are represented by mean±SD from at least three independent experiments. “***” represents “p<0.001” vs control group.

Article Snippet: Mouse prostate cancer cells RM-1 and mouse primary normal prostate basal epithelial cells were obtained from iCell Bioscience Inc. in Shanghai.

Techniques: Migration, Enzyme-linked Immunosorbent Assay, Cell Culture, Control

Journal: Cancer Management and Research

Article Title: Normal Basal Epithelial Cells Stimulate the Migration and Invasion of Prostate Cancer Cell RM-1 by TGF-β1/STAT3 Axis in vitro

doi: 10.2147/CMAR.S303122

Figure Lengend Snippet: Basal epithelial cells secreted TGF-β1 to promote the migration and invasion of RM-1. ( A ) Basal epithelial cells and TGF-β1 (5 ng/mL) changed the morphology of RM-1 from cuboid to spindle-shape. ( B ) HE staining and immunocytochemistry with anti-63 in mixed-cultured group. Arrowhead indicates basal epithelial cell of which p63 is positive. ( C ) Basal epithelial cells and TGF-β1 regulated the proteins associated with EMT. ( D ) Quantification of relative proteins expression in ( C ). ( E ) Effect of different concentration of SB431542 on RM-1 death was measured by CCK8 assay. SB431542 (2000 nM, 4000 nM) inhibited the migration ( F and G ) and invasion ( H and I ) of co-cultured RM-1. ( J ) Expressions of EMT relevant proteins were down-regulated when SB431542 (2000 nM or 4000 nM) was added into medium of co-cultured cells. ( K ) Quantification of relative expressions of proteins in ( J ). Values are represented by mean±SD from at least three independent experiments. “*” represents “p<0.05”. “**” represents “p<0.01”. “***” represents “p<0.001”.

Article Snippet: Mouse prostate cancer cells RM-1 and mouse primary normal prostate basal epithelial cells were obtained from iCell Bioscience Inc. in Shanghai.

Techniques: Migration, Staining, Immunocytochemistry, Cell Culture, Expressing, Concentration Assay, CCK-8 Assay

Journal: Cancer Management and Research

Article Title: Normal Basal Epithelial Cells Stimulate the Migration and Invasion of Prostate Cancer Cell RM-1 by TGF-β1/STAT3 Axis in vitro

doi: 10.2147/CMAR.S303122

Figure Lengend Snippet: TGF-β1 which was secreted by basal epithelial cells could promote migration and invasion of RM-1 depending on the phosphorylation of STAT3. ( A and B ) p-STAT3 was up-regulated by basal epithelial cells, conditional medium of basal epithelial cells and TGF-β1. ( C and D ) Expression of p-STAT3 in co-cultured group was down-regulated when SB431542 was added. ( E ) Stattic down-regulated the expression of p-STAT3 and STAT3. Stattic inhibited the proliferation ( F ), migration ( G ) and invasion ( H ) of untreated RM-1 and co-cultured RM-1. Values are represented by mean±SD from at least three independent experiments. “**” represents “p<0.01”. “***” represents “p<0.001”.

Article Snippet: Mouse prostate cancer cells RM-1 and mouse primary normal prostate basal epithelial cells were obtained from iCell Bioscience Inc. in Shanghai.

Techniques: Migration, Phospho-proteomics, Expressing, Cell Culture