Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

Article Title: Power-free microchip immunoassay of PSA in human serum for point-of-care testing.

doi: 10.2116/analsci.27.237

Figure Lengend Snippet: Fig. 2 Time course of the fluorescent intensity of the microfluidic immunoassay. The 0 min of amplification time in the horizontal axis is defined as the time from which both F-SA and b-anti-SA start flowing into the microchannel. PSA was diluted with PBS containing 1% BSA (a-1 and a-2) or human female serum (b-1 and b-2). Each data point is the average of four measurements obtained by four independent experiments. Additionally, fluorescence was obtained from LFDA (a-1 and b-1) or from no amplification (a-2 and b-2). The concentrations of PSA: blank (▼), 4.0 ng/mL (△), 100 ng/mL (■), 1.0 μg/mL (×), and 10 μg/mL (◣).

Article Snippet: Sample solutions of PSA were created by diluting PSA (Acris Antibody, Herford, Germany) either in PBS (pH 7.5, Cambrex Biosciences, Walkersville, MD) or in human female serum (Scipac, Kent, UK) to the desired concentrations.

Techniques: Amplification, Fluorescence

Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

Article Title: Power-free microchip immunoassay of PSA in human serum for point-of-care testing.

doi: 10.2116/analsci.27.237

Figure Lengend Snippet: Fig. 3 Calibration curve of PSA diluted in PBS (a-1 and a-2) or human female serum (b-1 and b-2). The error bars represent one standard deviation of four independent experiments. (a-1 or b-1) The curves were obtained from LFDA and the amplification time was 7.0 min. Insets magnify the graphs of the low concentration range. (a-2 and b-2) Calibration curve of PSA without amplification as control experiments. The time of detection was 4.0 min after the start of flow of F-SA.

Article Snippet: Sample solutions of PSA were created by diluting PSA (Acris Antibody, Herford, Germany) either in PBS (pH 7.5, Cambrex Biosciences, Walkersville, MD) or in human female serum (Scipac, Kent, UK) to the desired concentrations.

Techniques: Standard Deviation, Amplification, Concentration Assay, Control

Journal: Oxidative Medicine and Cellular Longevity

Article Title: HO-1 Is Essential for Tetrahydroxystilbene Glucoside Mediated Mitochondrial Biogenesis and Anti-Inflammation Process in LPS-Treated RAW264.7 Macrophages

doi: 10.1155/2017/1818575

Figure Lengend Snippet: TSG reduced RAW264.7 cells activation and proinflammatory cytokines release induced by LPS. RAW264.7 cells were exposed to various concentrations of TSG (0–480 μ M) for 6 hours. (a) Cell viability was analyzed by WST assay. (b) Annexin V-PI method was used to detect the cell death rate. (c-d) RAW264.7 cells were exposed to 1 μ g/mL LPS for 12 hours with or without TSG (120 μ M) pretreatment for 6 hours. Then, the morphological change was captured under a microscope. Bar = 50 μ m (×30). The percentage of activated cells in total cell population was calculated. (e-f) In the presence and absence of TSG (0, 30, 60, and 120 μ M) or Dex (100 μ M) for 6 hours, RAW264.7 macrophages were stimulated with 1 μ g/mL LPS for 12 hours. The concentration of TNF- α and IL-6 production in the culture medium were assessed by specific ELISA kits. All data were expressed as the means ± SEM of three independent experiments. ∗ P < 0.05 and ∗∗∗ P < 0.001 versus control; # P < 0.05 versus LPS treatment group.

Article Snippet: Mouse specific ELISA kit for IL-6 and TNF- α level was purchased from CUSABIO Biotechnology Corp. (Wuhan, China).

Techniques: Activation Assay, WST Assay, Microscopy, Concentration Assay, Enzyme-linked Immunosorbent Assay, Control